Fig. 1. Cell surface expression of CD41 (x axis) and CD34 (y axis) in yolk sac, para-aortic splanchopleure (P-Sp), aorta-gonad-mesonephros (AGM), fetal liver and adult bone marrow. (A) Isotype antibody staining of E7.0 whole embryo tissue, (B) E7.0 whole embryo tissue, (C) E8.0 yolk sac cells, (D) E9.5 yolk sac cells, (E) E9.5 P-Sp cells, (F) E10.0 yolk sac cells, (G) E10.0 AGM cells, (H) E12.5 fetal liver cells, and (I) adult marrow cells. Numbers listed in upper right corner are the percentage of total cells coexpressing CD41 and CD34 in each tissue. Representative gates used to identify CD41^lo/-, CD41^eim, and CD41^bright cells are depicted in panel D. Results are representative data of 4 experiments.