Fig. 2. Colony morphology and analysis of hemoglobin mRNA expression.
Photomicrograph of an E8.5 yolk sac primitive erythroid cells (EryP) and a
definitive erythroid progenitor cell (BFU-E). Magnification x100. Panels
to the right of the photomicrographs depict the results of RT-PCR analysis of
plucked erythroid colonies and reveal that EryP contain mRNA for
ßH1 (embryonic hemoglobin) and ß globin major (adult hemoglobin)
while BFU-E express mRNA for only ß globin major. Negative control
samples were tested using no reverse transcriptase.