Fig. 5. In situ hybridization showing LePHAN, LeYAB B and KNOX expression patterns in the SAM. (A-F) Wild type, (G-K) w6, (L-O) w3 and (P-S) w. mRNA accumulation patterns for LePHAN (A,B,C,G,L,P), LeYAB B (D,H,I,M,Q), LeT6 (E,J,N,R) and TKN1 (F,K,O,S). (A-C) LePHAN expression in the wild-type SAM and young leaf primordium (A), later stage leaf primordium (B) and leaflet primordia (lp; C). (G,L,P) LePHAN expression in the SAM and young leaf primordium of w6 (G) w3 (L) and w (P). Downregulation of LePHAN was seen in later leaf primordia in w6 (G inset), w3 (L inset) and w (P inset). (D) LeYAB B expression in the wild-type leaf primordium. (H,I,M,Q) YAB B was expressed in both adaxial and abaxial domains of leaf primordium in w6 (H,I), but only in adaxial domain in w3 (M), and no YAB B expression was detected in w leaf primordium (Q). (E) LeT6 expression in the wild-type SAM, leaf primordium and leaflet primordium (E inset). (J) LeT6 expression in the SAM and young leaf primordium in w6, and in later leaf primordia (J inset). (N,R) LeT6 expression in the SAM and the later stages of w3 (N, N inset) and w (R,R inset) leaf primordium. (F,K,O,S) TKN1 expression in wild type (F), w6 (K), w3 (O) and w (S). TKN1 expression in later stages of leaf primordium of w3 (O inset). Scale bars: 50 µm.