Fig. 5. A balance of E-cadherin is crucial for normal gonad formation. Anterior is leftwards. Stage 15 embryos carrying an enhancer trap (68-77) that expresses ß-GAL in SGPs. Embryos are labeled to reveal germ cells (anti-Vasa), the enhancer trap (anti-ß-GAL) and the cell surface of SGPs (anti-NRT). (A,A') Wild type. Germ cells are completely ensheathed by SGPs. Note that both ß-GAL and NRT staining is observed between germ cells. (A') NRT channel alone. (B,B') Embryo expressing two copies of a UAS-DE-cadherin transgene in the germ cells (nos-Gal4). Germ cells are tightly clustered inside the gonad, with no ß-GAL or NRT labeling between them. (B') NRT channel alone. Scale bar in A: 10 µm.