Fig. 6. foi is required for proper Drosophila E-cadherin levels in the gonad. Anterior is leftwards. (A) Stage 15 foi^20.71/foi^16.33 mutant. UAS-mCD8-GFP expressed in the mesoderm (twist-Gal4) labeled with anti-Vasa, anti-GFP and anti-NRT. The absence of SGP-derived GFP and NRT labeling around the germ cells indicates a failure in germ cell ensheathment. (B,C) foi^20.71 homozygous mutants. Embryos labeled with anti-Vasa, anti-EYA and anti-Drosophila E-cadherin. Gonad boundaries indicated with broken line. (B) Drosophila E-cadherin staining is reduced in the SGPs of foi mutants at stage 13 compared with wild type (Fig. 3). (C) Stage 15 male. The SGPs and germ cells have not compacted into a coalesced gonad but the msSGPs (C, arrow) have joined the gonad. Drosophila E-cadherin expression remains low in the SGPs and germ cells, but is high in the msSGPs (arrow). Nearby tissues (t, trachea) are not affected. Scale bar in A: 10 µm.