Fig. 2. Kette binds to actin. (A) Soluble Kette protein was prepared as a maltose-binding-protein (MBP) fusion and stayed in the supernatant (S) when centrifuged at 200,000 g for 30 minutes. By contrast, F-actin was found in the pellet (P). When soluble Kette is added to F-actin, about 50% of Kette associates with the F-actin. Proteins were separated by SDS-PAGE followed by Coomassie blue staining. (B) MBP does not bind to F-actin. (C) Kette interacts with G-actin. Four clones of highly related actin sequences were identified in a yeast two hybrid screen. Sequence analyses indicate that interaction with Kette occurs at the C terminus.