Fig. 3B. MMP9 expression, fibrin accumulation, and vascularization in implantation sites from galardin-treated wild-type and Plg-deficient mice. (B) Expression of MMP-9 at 8.5 dpc was detected by in situ hybridization (parts a-d) and was present in the giant trophoblast cells (black arrows) that are identified by their expression of PL-1 mRNA (red arrows) (parts e-h). The spatial distribution of the giant trophoblast cells in 8.5 dpc implantation sites from galardin-treated Plg-deficient mice (part d), was similar to that of mock-treated wild-type mice 7.5 dpc implantation sites (data not shown). Endothelial cells were detected by in situ hybridization for PECAM mRNA (parts i-l). The vascularization of the decidua in 8.5 dpc galardin-treated Plg-deficient mice (parts l,n) was like that found at 7.5 dpc mock-treated wild-type mice (Fig. 3A, parts i,m). The areas boxed in parts i and l are shown at higher magnification in parts m and n, respectively. am, antimesometrial; d, decidua; e, embryo; m, mesometrial. Scale bar: 500 µm in parts a-l; 100 µm in parts m,n.