Fig. 2. Expression domain of eyg in the adult mesothorax and the wing imaginal disc. (A) Adult mesothorax of a fly of genotype eyg-Gal4 > UAS-lacZ stained with X-Gal (blue). The eyg domain occupies most of the notum, except the scutellum (sc) and the lateral region, which includes some macrochaetes. The dorsocentral bristles are marked with arrows and the scutellar bristles with arrowheads. (B) Early (left) and mature (right) wild-type third instar wing discs stained for en (green) and eyg (red). The eyg expression domain occupies the greater part of the anterior compartment, but does not extend to the posterior compartment. Note the dot of expression in the wing hinge. (C) The thoracic region of a wild-type disc stained for neuralizer (neu; green), which marks the precursors of the macrochaetes, and eyg (red). The precursors of two dorsocentral bristles (arrows), as well as some of the pre-sutural ones, are inside the eyg domain. The scutellar bristles (arrowheads) and the more lateral ones are outside the eyg domain. (D) Double staining for pnr (blue) and eyg (red), showing the overlap of their expression domains. (E) Thoracic region of a wild-type wing disc doubly stained for Iro genes (green) and eyg, showing that the expression domains of the genes overlap in part.