Fig. 3. Phenotype of eyg mutants. (A) Adult thorax of an eyg^SA2/Df(3L) iro fly. The scutellum (sc) is present, as well as some of the lateral macrochaetes, but most or all the eyg domain is missing. Note the very low number of microchaetes, which compose most of the eyg domain (compare with Fig. 1A,B). (B,C) Anti-Eyg antibody of the thoracic region of a wild-type disc (B) and a eyg^SA2/eyg^20MD1-mutant disc (C). Low levels of anti-Eyg staining can still be observed in the mutant disc, suggesting that the antibody also recognises the Toe protein. (D) eyg expression at late embryogenesis of a wild-type embryo, as revealed by in situ hybridisation with a specific eyg probe. (E) In situ hybridisation using a specific toe probe reveals a similar expression pattern. (F) In eyg^SA2-mutant embryos there is no detectable eyg transcription. (G) In eyg^SA2-mutant embryos toe transcription appears normal. (H,I) Adult thorax of a fly of genotype eyg^Gal4-gv5/Df(3L)iro (H), showing a phenotype very similar to that of the fly in A. The high levels of toe activity in eyg^Gal4-gv5/Df(3L)iro > UAS-toe flies (I) give rise to a partial rescue of the mutant phenotype. Note the appearance of dorsocentral bristles and of numerous microchaetes.