Fig. 6. Regulation of eyg by the Iro and pnr genes. (AC) Large Iro gene-mutant clone (black; arrows) induced at first instar. The cells of the clone show no eyg expression, and the effect extends to wild-type cells close to the clone. (D-F) Thoracic region of a wing disc containing clones deficient for Iro gene activity (black). The medial region is to the left and the lateral is to the right. The clones located towards the lateral region (arrows) lose eyg expression (blue), whereas those located more medially (arrowheads) do not. (G) Wing disc of 638-Gal4 > UAS-ara showing that the ectopic ara expression induces eyg activity (red). (H) 638-Gal4 > UAS-pnr wing disc also showing activation of eyg by pnr.