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Fig. 5. The cytoplasmic domain of Kek1 is required for subcellular localization. (A-D) Confocal microscope sections showing anti-Myc immunostaining after overexpression of Myc-tagged UAS-kek1 and UAS-kek1TM in embryos (A and B, respectively) and wing imaginal discs (C and D, respectively) by using en-Gal4 as a driver. Apicobasal polarity is shown (a{leftrightarrow}b) with apical orientation upwards. Apical surface of wing imaginal discs is revealed with rhodamine-phalloidin. Anti-Myc staining is in green and rhodamine-phalloidin is in red. (E,F) Resulting UAS-kek1/en-Gal4 and UAS-kek1TM/en-Gal4 adult wings (compare with a wild-type wing in Fig. 2I).