Fig. 7. Northern analysis of RFX4 transcript expression using transcript-specific probes. cDNA probes corresponding to multiple or single RFX4 transcript variants, as indicated on the bottom of the figure, were used to probe northern blots containing total cellular RNA from adult testes (T), liver (L) and brain (B), or from brains of E18 mice of the +/+, +/- and -/- genotypes, as indicated. The blots were aligned to demonstrate the positions of the three hybridizing RFX4 species v1, v2 and v3 (arrows), as well as an uncharacterized transcript seen in adult mouse liver. There was no detectable hybridization of the specific v1 and v2 probes to the E18 brain RNA of any genotype (not shown).