Fig. 8. Developmental expression of RFX4_v3. (A-E) Whole-mount embryos at the indicated embryonic days (E) in which the RFX4_v3 transcript is indicated by the blue digoxigenin staining. (C) Whole-mount suggests minimal staining rostral of the zona limitans (zl); however, a section through the plane indicated as C' shows staining of the dorsal cortex (cx). (D,E) Wholemounts at E10.5; (F) midline sagittal section; (G-I) coronal sections through similar embryos. The arrowheads in F-H indicate the lost expression in the telencephalic dorsal midline at E10.5. (J-M) One sagittal (J) and three rostral-to-caudal coronal sections through the head at E12.5. Note the lack of staining in the telencephalic dorsal midline (arrowheads in J,K) in the epiphysis (ep) in L and in the fourth ventricle choroid plexus (ch) in M. Scale bars: 500 µm. mb, midbrain; fb, forebrain; hb, hindbrain; te, telencephalon; me, mesencephalon; rb, rhombencephalon; sc, spinal cord di, diencephalon; cb, cerebellum; cp/lt, commissural plate/lamina terminalis; LGE, lateral ganglionic eminence; MGE, median ganglionic eminence; ch, choroid plexus; R, retina; os, optic stalk; DT, dorsal thalamus; VT, ventral thalamus; HY, hypothalamus; V, trigeminal ganglion; VII/VIII, facial/vestibular ganglion.