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Fig. 3. Growth in a liquid environment promotes vulval induction. Scale bars: 20 µm. Animals are L4 stage hermaphrodites. (A) A wild-type animal displaying normal vulval induction that developed on standard NG plates. (B) A let-23(sy1) mutant animal displaying a vulvaless phenotype that developed on standard NG plates. (C) A let-23(sy1) mutant animal displaying normal vulval induction that developed in a liquid M9 environment.