Fig. 3. Immunohistochemical analysis of the cranial and trunk neural crest cells cultured in the presence of FGF2 and in combination with BMP2 and TGFß1. The bottom row shows cranial crest cultures infected with the virus containing the constitutively active (stabilized) ß-catenin. Panels show some of the most representative cultures for each culture condition. Specialized cell types are arranged in columns, whereas rows depict different growth conditions. Note that chondrogenesis is induced in the presence of exogenous FGF2 but is suppressed when BMP2 or TGFß1 are also added. Scale bars: 100 µm.