Fig. 5. The effect of FGF2 and other cytokines on cell differentiation of the cranial and trunk neural crest cells in vitro. This histogram of the results described in Fig. 3 compares effects of the cytokines on generation of pigment, glial, smooth muscle, neuronal cells and chondrocytes. As most of the cultures were stained with only two or three different cell-specific antibodies, the histogram represents percentage of independent cultures displaying a particular differentiated cell type from the total number of cultures stained for that marker. Chondrogenesis is inhibited by both BMP2 and TGFß1 proteins even in the presence of FGF2/8. The total number of cultures tested for each of the cell types is indicated above the representative bars. Note that all cultures were scored for melanogenesis.