Fig. 7. Immunohistochemical analysis of the cranial neural crest cultures infected with the RCAS::Hoxa2 and RCAS::Hoxd10 viruses and comparison with trunk neural crest culture in medium containing the exogenous purified FGF2 or BMP2 protein. All antibodies used are the same as shown in Fig. 3. In RCAS::Hoxa2-infected cells, the clusters of melanocytes, glial and smooth muscle cells form similarly to the FGF2 cultures; however, the ColII-positive cells form significantly smaller clusters and in fewer cultures. No chondrocytes are detected in Hoxd10-infected cultures, a condition that mimics the trunk neural crest cultures. Scale bars: 100 µm.