Fig. 4. Inhibition of polar body formation in zero-Na+ artificial SW
after 1-MA addition. GVBD occurred 20 minutes after 1-MA addition both in
normal SW and zero-Na+ artificial SW. In normal SW, the first and
second polar body (arrowheads) were extruded 70-90 minutes and 100-120 minutes
after 1-MA addition, respectively. In zero-Na+ artificial SW, polar
bodies were not extruded.