Fig. 8. Effect of LPP3 in Wnt target gene expression. Expression analysis of Wnt target genes during EB differentiation. Semi-quantitative RT-PCR analysis of markers at the indicated days in culture. The loading control was the Hprt gene. Bmp4 did not show significant differences in expression during EB differentiation. In contrast, while brachyury expression (a direct transcriptional target of the Wnt signaling pathway) decreased after 6 days in culture in heterozygous EBs, its expression was increased and prolonged in LPP3^-/- EBs.