Fig. 5. Adamts20 expression is distinct and precedes that of melanoblast marker DCT. Adamts20 expression (B,D,F,H,J) is compared with that of melanoblast marker DCT (A,C,E,G,I) through developmental stages E9.5 (A,B), E10.5 (C,D), E11.5 (E,F), and E12.5 (G-J). Adamts20 expression in the neural tube is visible at E9.5 (B), E10.5 (D) and E11.5 (F). At E9.5, DCT is expressed only the developing pigmented retinal epithelium of the eye (A) and in migrating melanoblasts at E10.5 (C). By E11.5, Adamts20 expression has extended laterally from the neural tube along the sides of the embryo with increased density surrounding the base of the limb buds E11.5 (F) and remains expressed here through to E12.5 (H,J). This expression of Adamts20 at the base of the limb buds (black arrows in E,F) at E11.5 precedes expression of DCT detected in migrating melanoblasts in the same region, visible at E12.5 (G,I), but not at E11.5 (E). Scale bars: 1 mm in A-D; 2 mm in E-J.