Fig. 5. Sog is cleaved by Tld in a Dpp-dose dependent fashion. (A) Sog, Tld and the indicated amount of Dpp were incubated for 4 hours at 25°C and Sog fragments were analyzed by western blotting using anti-Sog-CR1 or anti-Myc antibody. Processed fragments were detected at 3x10^-10 M of Dpp. (B) Sog, Tld, Tsg and the indicated amount of Dpp were incubated for 4 hours at 25°C, and Sog fragments were analyzed as above. Tsg was used at the same concentration as Dpp. Cleavage fragments were observed at 10^-10 M of Dpp. Arrows a-g show the same molecular weight fragments of Sog as shown in Fig. 3.