Fig. 6. Time course assay of Sog processing. (A) Sog, Tld and Dpp (3x10^-10 M) were incubated at 25°C for the indicated time, and Sog fragments were analyzed on western blots using anti-Sog-CR1 or anti-Myc antibodies. The processed fragments were barely seen at 240 minutes. (B) Sog, Tld, Dpp (3x10^-10 M), and Tsg (3x10^-10 M) were incubated in the same conditions as above. Processed fragments were detected with as little as 15 minutes of incubation. Arrows a-g indicate the same fragments of Sog shown in Fig. 3.