Fig. 1. Vascular pattern development in wild-type (A,B) and in fkd1 (C,D) cotyledons and mature cotyledons of wild type (E), fkd1 (F), fkd2 (G), fkd2 fkd1 (H), mp^G92 (I), mp^G92 fkd1 (J), axr6-2 (K) and axr6-2 fkd1 (L), viewed with phase contrast optics (A,C), DIC optics (B,D) and dark-field illumination (E-L). Cleared cotyledons (1 DAG) of wild type (A) and fkd1 (C), show the presence of provascular tissue of the midvein (mv), the distal secondary veins (ds), and occasionally proximal secondary veins (ps) that show partial maturation by 3 DAG (B,D) and complete maturation by 14 DAG (E,F). Arrows indicate freely ending veins and * in A indicates the initiation of a proximal secondary vein adjacent to the distal secondary vein. Scale bar: 50 µm.