Fig. 3. Loss of peripherin-positive type II afferent neurones in the cochleae of TrkB and Bdnf mutants. Cochleae were isolated, sectioned and stained with antibodies against peripherin as described under Materials and methods. Representative micrographs illustrating the presence of type II spiral ganglion neurones (SG) in the midbasal cochlear turn of the organ of Corti in adult TrkB^shc/+ (A) and P19 Bdnf^+/+ (C) control mice; no peripherin-positive neurones were detected in TrkB^shc/- (B) and Bdnf^-/- mutants (D). Scale bars: 50 µm.