Fig. 3. Effects of EcR-F645A expression in targeted tissues. (A-C) Effects on eye development. Adult eyes are shown from animals containing a GMR driver and (A) no responder, (B) an EcR-F645A responder, and (C) responders for both EcR-F645A and EcR-B2. (D) Effects on larval epidermis. The two animals are of equivalent age, and both contain an Eip71CD₆₅₇ driver; the pupa on the left has no responder, the animal on the right has an EcR-F645A responder. (E,F) Effects on glue secretion. The two puparia are of equivalent age, and both carry a transgene expressing fluorescent glue (Sgs-GFP) (Biyasheva et al., 2001). They have an Sgs3 driver and either no responder (E) or an EcR-F645A responder (F). Note that the green fluorescence in E is entirely external to the puparial case. (G,H) Effects on fat body dissociation. Pupae containing the Lsp2 driver, a GFP.nls responder and either no EcR responder (G) or an EcR-F645A responder (H) were dissected and viewed by fluorescence; only the fat body cells are visible. (I,J) Effects on border cell migration. Stage 9 egg chambers were dissected from adult females containing the slbo driver, a GFP responder, and either no EcR responder (I) or an EcR-F645A responder (J). Arrows indicate border cells; o, oocyte. A-D were photographed under bright field illumination. G,H were photographed with GFP-fluorescence optics. E, F, I and J were photographed with a mixture of bright-field and fluorescence optics.