Fig. 5. Effects of targeted expression of individual EcR isoforms in the presence of EcR-F645A. Flies homozygous for both the EcR-F645A responder and a responder corresponding to the indicated wild-type EcR were crossed to each driver stock. EcR-C was not tested with the act5C and EH drivers. All other combinations of drivers were tested; if no bar is visible, there were no progeny with wild-type phenotype. For the EH driver, wild-type phenotype is expanded wings; for the other drivers, wild-type phenotype is survival to adulthood. In the case of the GMR driver, wild-type EcR isoforms restored wild-type eye morphology as well as viability (see Fig. 5C). Data are shown for crosses performed at 25°C, but the results were independent of temperature (data not shown).