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Fig. 3. En expression in the posterior A9 extends to the anterior A10-11. (A) En (green) and Hh (red) expression in A9 and A10-11 segmental primordia (AP) in the male genital disc. En expression in the posterior compartment of A9 segment extends to the A10-11 segment in a graded manner (double arrow, B). (C) En (green) and Cad (red) expression in A9 and A10-11 segmental primordia (AP) in the male genital disc. En and Cad expression overlap in the most anterior cells of the A10-11 segment. A gradient of both En and Cad expression is observed (double arrow) in the overlapping region (D). Hh expression therefore defines the border between segments A9 and A10.