Fig. 5. Cell lineage restriction in dsx¹ intersexual genital discs. (A,B) ß-Gal-expressing clones (red) in dsx¹ intersexual genital discs induced at 0-24 hours of development. (A) A ß-gal-expressing clone in the A8 segment that does not cross to the A9 segment. To distinguish the segmental border between male and female genital primordia, we used an antibody against Teashirt (green) that is specifically expressed in the A8 abdominal segment (female genital primordium). (B) Clones in A9 do not cross to A10-11. We used the anti-Cad antibody (green) to distinguish the segmental border between the male primordium and the anal primordium.