Fig. 9. Effect of ablation of the genital and anal primordium cells on the development of the genital disc. (A,B) Lack of terminal structures of female (A) and male (B) cad-GAL4; UAS-ricin, UAS-lacZ adults. (C) A very reduced cad-GAL4; UAS-ricin, UAS-lacZ genital disc. The anal primordium is labelled with anti-Cad antibody (in red). (D,E) Male genital discs expressing the cold-sensitive Ricin mutant protein in A8 (repressed female genital primoridum) using the tsh-GAL4 line at the restrictive temperature (D) where the Ricin protein is not functional, and (E) at the permissive temperature. The discs are stained with anti-ß-gal (in red) to label the A8 segment and with anti-Distal-less (in green) antibodies. Note the small size of the genital disc expressing functional Ricin protein in A8 and the lack of Distal-less expression in A9 (male genital primordium). Broken lines separate the three segmental primordia of the genital disc. T5, tergite 5; T6, tergite 6; T7, tergite 7.