Fig. 3. Expression pattern of the C3G gene. (A-D) ß-galactosidase reporter activity in whole heterozygous (A) and wild-type E11.5 embryo (B), and in a 16 µm paraffin sagittal section of an E11.5 heterozygote embryo (C) and in a small cephalic blood vessel at E12.5 (D). (E-L) Radioactive in situ hybridisation using an antisense C3G riboprobe (E,F, probe 2, Fig. 1A) and a sense control probe (G,H) on paraffin sections of E12.5 wild-type embryos and the antisense probe on sections of an E11.5 wild type (I,J) or homozygous mutant embryo (K,L). Ubiquitous expression of the C3G locus is shown by the reporter activity and by in situ hybridisation. Note specifically presence of reporter activity and endogenous gene activity in all cells including blood vessel endothelial cells and blood vessel surrounding cells in both small (D,J) and large blood vessels (I). Also note the absence of silver grains in the sections of homozygous embryos (K,L). Arrows point to blood vessel surrounding cells, arrowheads to endothelial cells (D,I,J). Bar represents 70 µm in A,B,C,E,F,G,H and 10 µm in I,J,K,L.