Fig. 9. Integrin ß3 and ß1 distribution in C3G mutant MEFs cultured with serum. (A-D) Integrin ß3 distribution was unaffected by the C3G mutation in the absence (A,B) or the presence (C,D) of serum. Integrin ß3 foci were more numerous and smaller than integrin ß1-positive foci (D,F). (E-K) We investigated integrin ß1 and paxillin distribution in MEFs plated in the presence of serum, because the morphology of C3G mutant MEFs was highly abnormal without serum on all substrates (see Fig. 8). Although C3G mutant MEFs appeared healthy when plated onto gelatine-coated cover slips in the presence of serum, integrin ß1 foci were similarly reduced in number (compare E and F) as in culturs without serum (compare Fig. 8A with B and 8G with H). Only very few integrin ß1-positive foci coincided with paxillin staining (J, yellow) as compared to controls (K). Arrowheads point to examples. Bar represents 14.5 µm in all panels.