Fig. 2. Olfactory structures develop normally in Ebf2-null mice. Histochemical staining conducted on vibratome sections of E12.5 (A) and E13.5 (B)mice. lacZ expression can be observed in the olfactory epithelium (oe), in the vomeronasal organ (vno) and alongside olfactory/vomeronasal fibers (arrows). (C,D) Peripherin-positive fibers connecting the olfactory epithelium to the hypothalamus (arrows) are normally distributed in wild-type (C) and mutant (D) embryonic brains alike (arrowhead indicates fibers in the nasal mesenchyme). (E,F) Nissl staining of P30 paraffin coronal sections. The mutant olfactory bulb (F) is histologically indistinguishable from the wild-type one (E). cp, cribriform plate; epl, external plexiform layer; gl, glomerular layer; gr, granule cell layer; hy, hypothalamus; ipl, internal plexiform layer; lot, lateral olfactory tract; m, mitral cell layer; mot, medial olfactory tract; ns, nasal septum; oe, olfactory epithelium; vno, vomeronasal organ. Scale bars: 100 µm in A; 200 µm in B; 150 µm in C,D; 100 µm in E,F.