Fig. 3. Migrating GnRH-neurons express Ebf2. Arrowheads in A-C indicate cell bodies of migrating neurons. (A) GnRH-like immunostaining in Ebf2^-/- neurons migrating dorsally through the nasal mesenchyme at embryonic day 15. (B) Anti ß-galactosidase staining in the same neurons. (C) Superimposed signals from A and B revealed co-expression of Ebf2 and GnRH in migrating neurons. (D) Hoechst nuclear counterstaining of same field. As described (Wray, 2001), GnRH-neurons migrate through cell-poor, nerve fiber-rich regions. Scale bar: 20 µm.