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Fig. 5. Hypoplasia of seminipherous tubules and reduced spermatogenesis in Ebf2-null mice. (A,C,E) Wild type; (B,D,F) Ebf2-/-. (A,B) Hematoxylin and Eosin (HE) staining of seminipherous tubules. In B, the luminal side of the tubule contains very few aggregations of spermatids compared with wild-type tubule. (C,D) Anti BrdU immunostaining reveals a sharply reduced number of proliferating spermatogonia in mutant tubules (arrows). (E,F) HE-stained epidydimes. The lumen of a mutant epidydimis (F) contains cell debris and hardly any sperm cells. The latter are recognizable as Hematoxylin-stained (dark purple) dots in the normal control (E). Scale bar: 40 µm.