Fig. 6. The signature motif for glycosyltransferases is necessary for the Nti activity. Notch-Delta binding was assayed with Nti-G3-myc that was created from Nti-myc by inducing amino acid changes ERD (shown by # in Fig. 2A) to GGG. ERD is thought to be important for glycosyltransferase activity. The analysis was done and the results were presented as in Fig. 4A. Compared to the binding observed in Nti-myc, Notch and Fng co-expressing cells, Nti-G3-myc cotransfection with Notch and Fng does not enhance Notch-Delta binding. Instead Nti-G3-myc acts as dominant-negative form of Nti, because the Notch-Delta binding is lower than observed in Notch and Fng co-transfected cells.