Fig. 2. Seed coat, seedling and flower phenotypes of mutants and transformants. (A-I) Scanning electron micrographs of seed coats illustrating the columella phenotypes. (J-L) Ruthenium red-stained seed coat mucilage phenotypes. (M-O) Seed coat pigment phenotypes. (P-R) 5-day-old seedlings. (S,T) First and second leaf stage seedlings. (U) Single flowers. (A) gl3-1 single mutant (looks like wild type). (B) gl3-1 egl3-1 double mutant. (C) egl3-1 single mutant. (D) tt8 single mutant (looks like wild type). (E) egl3-1 tt8 double mutant. (F) egl3-1 gl3-1 tt8 triple mutant. (G) ttg1-1. (H) CaMV35S::EGL3 in ttg1-1. (I) CaMV35S::GL3 in ttg1-1. (J) gl3-1 single mutant (looks like wild type); (K) gl3-1 egl3-1 double mutant (looks like egl3-1 single mutant); (L) egl3-1 tt8 double mutant. (M) Left: wild type; right: ttg1-1 mutant. (N) Lower left: CaMV35S::GL3 in ttg1-1; lower right: CaMV35S::EGL3 in ttg1-1; upper center: hybrid expressing CaMV35S::GL3 and CaMV35S::EGL3 in ttg1-1. (O) Lower center: tt8; upper left: CaMV35S::GL3 in tt8; upper right: CaMV35S::EGL3 in tt8. (P-R) Seedlings in order left to right: (P) wild type, ttg1-1, gl3-1, egl3-1, gl3-1 egl3-1 double mutant; (Q) ttg1-1, CaMV35S::GL3 in ttg1-1, CaMV35S::EGL3 in ttg1-1, hybrid expressing CaMV35S::GL3 and CaMV35S::EGL3 in ttg1-1; (R) tt8, CaMV35S::GL3 in tt8, CaMV35S::EGL3 in tt8. (S) CaMV35S::GL1 in wild type. (T) Hybrid expressing CaMV35S::GL1 and CaMV35S::EGL3 in wild type. (U) Left: PAP1-D flower (wild type expressing CaMV35S::PAP1, activation tagged); right: flower of hybrid coexpressing CaMV35S::PAP1 and CaMV35S::EGL3. Arrows in the first three panels point to examples of columellae types: C, normal round-topped columella; CC, collapsed columella; PCC, partially collapsed columella; MC, missing columella; PM, patchy mucilage; MM, missing mucilage.