Fig. 5. Reduction of Fgf10 expression in Hhip (Hip1 in figure) mutant pancreas. Whole-mount in situ hybridization of Fgf10 expression in E10.5 (A,B) and E11.5 (C,D) pancreatic tissue in control (A,C) and Hhip^–/– embryos (B,D). Fgf10 expression (blue) was attenuated in Hhip^–/– mutant pancreas bud at E10.5 (B); however, Fgf10 levels were recovered at E11.5 (D). Staining of control and mutant tissues was performed in parallel to detect quantitative differences in Fgf10 expression. d, duodenum; db, dorsal pancreas bud; lb, lung bud; st, stomach; vb, ventral pancreas bud.