Fig. 2. Telencephalic expression of Gsh2 and Nkx2.1 at 36-38 somites. Expression of Gsh2 (green) and Nkx2.1 (red) on coronal telencephalic sections is shown from anterior (A) to posterior (F). (A) In the anterior telencephalon, Gsh2 is expressed in the lateral domain; Nkx2.1 is not expressed at this level. (B) The most anterior expression of Nkx2.1 is at the level of the septum, where Gsh2 expression is non-overlapping with Nkx2.1 expression. (C-E) More posteriorly, some Gsh2-positive cells (yellow) begin to be observed within the Nkx2.1-positive domain. (F) At the most posterior level of the telencephalon, Gsh2 is highly expressed in the region of the presumptive CGE, whereas Nkx2.1 is expressed only in the diencephalon. CGE, presumptive future caudal ganglionic eminence; Di, diencephalon; Sep, septum. Scale bar: 50 µm (A-F).