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Fig. 8. Reduction of cortical interneurons in Nkx2.1–/–;Gsh2–/– mutants. E18.5 coronal sections were immunostained for the interneuronal markers Calbindin and GABA. A marked reduction in both Calbindin-positive (A,B) and GABA-positive cortical interneurons (C,D) is observed in Nkx2.1–/–;Gsh2–/– mutants (B,D) compared with controls (A,C). Arrows show individual interneurons. Scale bar: 25 µm (A-D).