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Fig. 1. Counting peripodial membrane cells. Wing discs of different genotypes were stained for Armadillo and large peripodial cells were counted on a laser-scanning confocal microscope at 60x magnification. (A) A single optical section of only peripodial membrane. Such optical sections were used to obtain a relative count of peripodial cells in different genetic backgrounds. (B) A single optical section of disc proper. (C,D) vg-GAL4 (C) and 426-GAL4 (D) expression patterns as seen with UAS-lacZ. (E,F) vg-GAL4/UAS-Ubx (E) and 426-GAL4/UAS-Vg (F) wing discs reconstructed from several optical scans showing large peripodial and small disc proper cells.