Fig. 2. Genes/enhancer-trap lines expressed in the wing disc peripodial membrane. (A-D) lio-lacZ (A), odd-GAL4/UAS-nuclear lacZ (B), coro-GAL4/UAS-nuclear lacZ (C) and en-GAL4/UAS-nuclear lacZ (D) wing discs stained with anti-ß-galactosidase antibodies. (E) Wild-type wing disc stained with anti-Ubx antibodies. coro-GAL4 and Ubx are not expressed in the DP, whereas only Ubx is expressed in all PM cells. E also shows spatial domains of the DP. P, pouch; H, hinge, LN, lateral notum; MN, medial notum. (F-J) Ubx-GAL4 expression patterns during different stages of development. The expression of Ubx-GAL4 was detected by either anti-ß-gal antibody staining (F,G,I,J) or X-gal staining (H). (F) In the wing disc, Ubx-GAL4 is expressed (green) only in the peripodial membrane. The disc proper is stained with anti-Wg antibody (red). (G) Stage 15 embryo showing lacZ expression in T2, T3 and in all abdominal segments. (H,I) Mid- to late second instar wing discs, showing PM-specific expression of Ubx-GAL4. In I, the disc proper is stained with anti-Wg antibody (red), which is expressed in the anterior-ventral quadrant of second instar discs. (J) Ubx-GAL4 expression pattern in the haltere disc. It is expressed in the disc proper as well as in the peripodial membrane. This GAL4 driver is predominantly expressed in the entire anterior compartment of the disc.