Fig. 6. Pattern duplications observed in flies over-expressing DNDER, DN-RAS, DN-Raf and Aos in the PM reflect notum/hinge-to-wing transformations in wing discs. (A-C) Ubx-GAL4/UAS Dras1^N17 (A), Ubx-GAL4/UAS-DN-Raf (B) and Ubx-GAL4/UASAos (C) wing discs stained for Wg. Note mesonotum-to-wing blade transformation as indicated by the over-growth and extension of the DV boundary to the mesonotum region (arrowheads). Larvae with such high degree of notum-to-wing transformation are invariably early pupal lethal. (D,E) Ubx-GAL4/UAS-Dras1^N17 wing discs double stained with Ci and Wg (D), and En and Wg (E) to show pattern duplications (arrowheads). (F,G) Ubx-GAL4/UAS-Dras1^N17 (F) and Ubx-GAL4/UAS-Aos (G) wing discs stained for Ubx. Note that Ubx expression in the PM is not altered in these discs. The figure also suggests that the identity of PM cells is not changed. (H,I) Ubx-GAL4/UAS-Dras1^N17 (H) and Ubx-GAL4/UAS-Aos (I) wing discs stained for Arm. Only transformed part of discs are shown at higher magnification. Note that PM cells in these experiments have retained their normal morphology (arrowheads).