Fig. 4. Na⁺/K⁺ ATPase localization to the septate junction depends on Nrv2 but not Coracle. In wild-type (A,B,C) and coracle mutant animals (E), the Na⁺/K⁺ ATPase (red A,C,E) localizes to the septate junction marked by Coracle in wild-type animals (green A,B). However, in nrv2 mutants (D) Na⁺/K⁺ ATPase staining is reduced and/or not localized to the septate junction. Na⁺/K⁺ ATPase staining (5 anti- subunit monoclonal) is undetectable in ATP mutants (data not shown). Basal surfaces are outlined in white, apical/lumenal surfaces in blue. Scale bar: in E'', 5 µm for A-E,A''-E''; in E', 5 µm for A'-E'.