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Fig. 1. Specific inhibition of twisted gastrulation and chordin translation using antisense morpholino oligonucleotides. (A) Sequences of the TSG-MO and two chordin MOs used in this study and their relative positions to translation start sites on their respective mRNAs. (B) The TSG-MO, but not chordin MOs, inhibits translation of TSG from the intact TSG mRNA, but not from a TSG mRNA that contains a modified sequence upstream of the TSG translation start site (BM40-XTSG1: see Materials and methods). (C) Both ChdA-MO and ChdB-MO completely block the translation of chordin RNA that contains their respective complementary starting sequences, and they also mutually reduce the translation of chordin from the other allelic RNA. Chordin MOs neither inhibit the translation of a chordin mRNA that lacks the 5'-UTR ({Delta}5'-Chd), nor do they interfere with the translation of TSG. Arrows in B,C indicate the XTSG1 and chordin proteins, respectively.