Fig. 6. Growth cone morphology analysis for POC axons. Growth cone morphology can be visualized during timelapse imaging of the POC axons. (A-C) Representative growth cones of leader (long pink arrow) and follower (long blue arrow) axons are shown. Filopodia present on both types of growth cones are also indicated with smaller arrows (pink for leader and blue for follower). Scale bar: 10 µm. (D) Average growth cone areas do not differ between leader (n=12) and follower (n=8) axons. Only clearly visible follower axons were chosen for this analysis. (E) Average width to length (w/l) ratio plot shows a significantly higher ratio for leader axons (n=12) compared with follower axons (n=8) (P<0.05, Student's t-test).