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Fig. 5. Effects of glo1-1 on basal endosperm-specific gene expression. mRNA in situ analysis using (A-C) ZmESR and (D,E) a BETL-specific marker (J.F.G.-M., unpublished). (A-C) ZmESR expression (shown in purple, arrows) in (A) 7 dap wild-type endosperm, and in glo1-1 endosperms at (B) 7 dap and (C) 10 dap. BETL-specific gene expression in 10 dap (D) wild-type BETL and (E) glo1-1 basal endosperm, where expression is confined to a single cell (arrow). pBET1-GUS expression (pink) in (F) wild-type and (G) glo1-1 endosperms at 14 dap. Scale bars: 100 µm.