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Fig. 1. Inhibition of {gamma}-secretase in cultured metanephroi eliminates Notch activation and reduces tissue complexity. (A,E) Activated Notch1, detected with an antibody against the antigen generated by {gamma}-secretase (VLLS) (Schroeter et al., 1998), is present in the nucleus of S- and comma-shaped bodies (red circles) in 14.5 dpc embryonic kidney. (E) Control with no primary antibody. (B-D) Metanephroi cultured in DMSO-containing media or (F-H) 1 µM DAPT-containing media. NICD is present in control (B) but is undetectable after 5 days in DAPT (F). After the first day of culture, phase contrast images of control (C) and DAPT-treated metanephroi (G) are indistinguishable. After 5 days, the control metanephros is filled with high contrast structures (D), while the DAPT-treated contralateral metanephros has branches but less tissue complexity (H).