Fig. 2. Linkage between tbx1 and vgo^tm208. (A) Amino acid sequence alignment of zebrafish tbx1 and other T-box genes. Residues identical to zebrafish tbx1 are in gray. Only T-domain sequences are shown. (B) Physical and genetic maps of linkage group 5 (LG5) showing map positions of tbx1 and vgo^tm208. (C) Sequence analysis of tbx1 in wild-type (wt), vgo^tm208 and vgo^tu285 mutants. Box diagrams represent conceptual products of translation of tbx1 cDNAs from wild type, vgo^tm208 and vgo^tu285. T-box region is shown by hatched boxes. Note that the A to T transition in vgo^tm208 eliminates the AlwNI recognition sequence (CAGNNNCTG).