Fig. 7. Rescue of edn1 expression by transplanted endodermal cells in vgo/tbx1 embryos. Transplanted cells in brown (labeled with biotin) and edn1 expression in blue. (A) Expression of edn1 in a 27 hpf wild-type control embryo. (B) vgo/tbx1 host embryo in which transplanted wild-type cells induced upregulation of edn1 (arrows). (C) vgo/tbx1 host embryo, in which Tar*-injected endodermal cells contributed to the pharynx on only one side. On the control side, edn1 expression is weak and disorganized. The side that received endodermal cells (brown cells, arrows) shows much stronger expression of edn1, which includes cells located several cell diameters away from the transplanted cells (arrowheads point at pigment cells). Flat mounts in dorsal views with anterior towards the left in all panels.