Fig. 2. The Minute technique. The homozygous mutant cells produced by mitotic recombination lack the Minute mutation, and thus divide more quickly than the surrounding heterozygotic cells. The homozygous Minute clone usually dies. The photograph shows a wild-type clone, lacking GFP (green), in a +/RpS5² [also known as Minute(1)o^sp] late third instar wing imaginal disc.